Frizzled-5 rabbit pAb

• Catalog Number: orb770435
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Frizzled-5
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human FZD5. AA range:461-510
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 65
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-FZD5 antibody, anti-C2orf31 antibody, anti-Fr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of A549 cells, using FZD5 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from LOVO cells, using FZD5 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).