Cart summary

You have no items in your shopping cart.

Anti-Frizzled 5 Antibody

Catalog Number: orb214762

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb214762
CategoryAntibodies
DescriptionRabbit polyclonal antibody to FZD5
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Frizzled 5. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000, IHC-P: 1-100-1-200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetFZD5
Entrez7855
UniProt IDQ13467
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti C2orf31 antibody, anti Frizzled-5 antibody, a
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-Frizzled 5 Antibody

Western blot analysis of Frizzled 5 expression in A375 (A), mouse kidney (B), rat kidney (C), rat heart (D) whole cell lysates. (Predicted band size: 64 kD; Observed band size: 65 kD)

Anti-Frizzled 5 Antibody

Immunohistochemical analysis of Frizzled 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Frizzled 5 Antibody

Immunofluorescent analysis of Frizzled 5 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

  • Frizzled-5 rabbit pAb [orb770435]

    ELISA,  IF,  IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    50 μl, 100 μl
  • Anti-Frizzled 5 Antibody [orb665816]

    IF,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    30 μl, 200 μl, 100 μl
  • Anti-SARP3 Antibody [orb668083]

    IF,  IH,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 200 μl, 100 μl
  • Anti-Frizzled 5 Antibody [orb737324]

    IF,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • SFRP5 Antibody [orb417927]

    ELISA,  IHC

    Human

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg