Anti-EZH2 Antibody

• Catalog Number: orb378050
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EZH2.
• Target: EZH2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant full length protein of human EZH2
• UniProt ID: Q15910, Q61188
• Tested applications: IH, IP, WB
• Dilution range: WB: 1:500-2000, IF/ICC: 1:20-100
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KMT6; Histone-lysine N-methyltransferase EZH2; ENX
• Note: For research use only
• Entrez: 14056, 2146

Western blot analysis of EZH2 expression in HL60 (A), SW620 (B), MCF7 (C), mouse ovary (D) whole cell lysates. (Predicted band size: 79; 81; 84; 85; 86 kD; Observed band size: 98 kD)

Immunohistochemical analysis of EZH2 staining in human thyroid cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunoprecipitation of EZH2 from 0.5 mg Hela whole cell extract lysate, using Anti-EZH2 Antibody.