EZH2 Antibody

• Catalog Number: orb1931591
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB23144
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: IF: 1:25, WB: 1:2000, WB: 1:2000, WB: 1:1000, WB: 1:2000, WB: 1:2000, WB: 1:500
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 85363 Da
• Target: This EZH2 antibody is generated from rabbits immunized with a recombinant fragment (N-term) protein from human EZH2.
• UniProt ID: Q15910
• NCBI: NP_001190178.1, NP_001190177.1, NP_694543.1, NP_001190176.1, NP_004447.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Histone-lysine N-methyltransferase EZH2, ENX-1, En
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human cervical epithelial adenocarcinoma cell line) cells labeling EZH2 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin at 1/100 dilution (red).

Western blot analysis of lysates from Jurkat, T47D cell line (from left to right), using EZH2 Antibody. Diluted at 1:2000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

Anti-EZH2 Antibodyat 1:500 dilution + MDA-MB-468 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 85 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from MDA-MB-468, SW620, T47D cell line, mouse spleen, mouse testis tissue (from left to right), using EZH2 Antibody. Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

All lanes: Anti-EZH2 Antibody at 1:2000 dilution. Lane 1: mouse testis lysates. Lane 2: MDA-MB-231 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 85 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-EZH2 Antibody at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: MCF-7 whole cell lysate. Lane 4: Neuro-2a whole cell lysate. Lane 5: T47D whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 85 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-EZH2 Antibody at 1:2000 dilution. Lane 1: Jurkat whole cell lysates. Lane 2: MDA-MB-231 whole cell lysates. Lane 3: MDA-MB-468 whole cell lysates. Lane 4: T47D whole cell lysates. Lane 5: mouse spleen lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 85 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.