EZH2 Antibody (Center)

• Catalog Number: orb1931592
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This EZH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from 410-440aa of human EZH2.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Other
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q15910
• MW: 85363 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, IHC-P: 1:50~100
• Antibody Type: Primary Antibody
• Clone Number: RB2921
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Histone-lysine N-methyltransferase EZH2, ENX-1, En
• Note: For research use only
• NCBI: NP_694543.1, NP_001190176.1, NP_004447.2, NP_001190178.1, NP_001190177.1

Formalin-fixed and paraffin-embedded human Testis tissue reacted with EZH2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The anti-EZH2 Ctr Pab is used in Western blot to detect EZH2 in mouse kidney tissue lysate.

Anti-EZH2 Antibody (I418) at 1:1000 dilution + C6 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 85 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.