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Anti-ERK1/2 Antibody

Catalog Number: orb214452

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb214452
CategoryAntibodies
DescriptionRabbit polyclonal antibody to ERK1/2
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat, Zebrafish
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human ERK1/2. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000, IF/ICC: 1-100-1-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetMAPK1; MAPK3
Entrez5594, 116590, 5595, 50689
UniProt IDP27361, P21708, P63086, P28482
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesAnti-ERK 1 antibody, anti-ERK 2 antibody, anti-ERK
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-ERK1/2 Antibody

Western blot analysis of ERK1/2 expression in mouse lung (A), rat lung (B) whole cell lysates. (Predicted band size: 43; 41 kD; Observed band size: 44 kD)

Anti-ERK1/2 Antibody

Immunohistochemical analysis of ERK1/2 staining in human tonsil cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-ERK1/2 Antibody

Immunofluorescent analysis of ERK1/2 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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