EGFR Antibody [KO/KD Validated]

• Catalog Number: orb665717
• Category: Antibodies
• Description: The EGFR Antibody [KO/KD Validated] is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EGFR. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EGFR. The exact sequence is proprietary.
• UniProt ID: P00533, Q01279
• Tested applications: IHC, WB
• Dilution range: WB: 1-500-1000, IHC-P: 1-50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERBB; ERBB1; HER1; Epidermal growth factor recepto
• Research Area: Cancer, Developmental Biology, Epigenetics, Infect
• Note: For research use only
• Entrez: 13649, 1956
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EGFR expression in HepG2 (A), mouse embryo (B) whole cell lysates. (Predicted band size: 134 kD; Observed band size: 175 kD)

Immunohistochemical analysis of EGFR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.