EGFR Antibody [KO/KD Validated]

• Catalog Number: orb256509
• Category: Antibodies
• Description: The EGFR Antibody [KO/KD Validated] is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EGFR. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EGFR. The exact sequence is proprietary.
• UniProt ID: P00533, Q01279
• Tested applications: IF, WB
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERBB; ERBB1; HER1; Epidermal growth factor recepto
• Research Area: Cancer, Developmental Biology, Epigenetics, Infect
• Note: For research use only
• Entrez: 13649, 1956
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EGFR expression in HCC827 (A), A549 (B), mouse lung (C), rat lung (D) whole cell lysates. (Predicted band size: 134 kD; Observed band size: 175 kD)