Anti-EGF Antibody

• Catalog Number: orb382607
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EGF
• Target: EGF
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EGF. The exact sequence is proprietary.
• UniProt ID: P01132, P01133
• Tested applications: IH, WB
• Dilution range: WB: 1:500:2000, IHC-P: 1:50:200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-Pro-epidermal growth factor antibody, anti-EG
• Note: For research use only
• Entrez: 1950, 13645
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EGF expression in K562 (A), Hela (B), HEK293T (C), NIH3T3 (D) whole cell lysates. (Predicted band size: 133 kD; Observed band size: 133 kD)

Immunohistochemical analysis of EGF staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.