EGF Antibody (Center)

SKU: orb1937113

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:25
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD38777
• Immunogen: This EGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-720 amino acids from the Central region of human EGF. Antigen Region: 690-720 aa.
• Target: EGF
• Molecular Weight: 133994 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pro-epidermal growth factor, EGF, Epidermal growth factor, Urogastrone, EGF

All lanes: Anti-EGF Antibody (Center) at 1:2000 dilution. Lane 1: M.stomach tissue lysates. Lane 2: A549 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 134 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

EGF Antibody (Center) western blot analysis in mouse stomach tissue lysates (35 ug/lane). This demonstrates the EGF antibody detected the EGF protein (arrow).

EGF Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of EGF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Staining EGF in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Staining EGF in Human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Quick Database Links

Gene Symbol

EGF

UniProt

P01133

RefSeq (Protein):NP_001954.2, NP_001171601.1, NP_001171602.1