ADGRE1 Rabbit Polyclonal Antibody

• Catalog Number: orb156726
• Category: Antibodies
• Description: ADGRE1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Guinea pig, Porcine, Rat
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ADGRE1 (701-800/886aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 95 kDa
• Target: ADGRE1
• UniProt ID: Q14246
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: F4/80; Adhesion G protein-coupled receptor E1; Cel
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Xuxin Chen 1, Fan Wang 1, Jian Tang 1, Jiguang Meng 2, Zhihai Han 3 Paralemmin-3 augments lipopolysaccharide-induced acute lung injury with M1 macrophage polarization via the notch signaling pathway Respir Physiol Neurobiol, 320, 104203 (2024)

PubMed: 38103708

Applications

FC

Reactivity

Rat

Blank control (black line): Raw264.7. Primary Antibody (green line): Rabbit Anti-ADGRE1 antibody (orb156726), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Recombinant human EMR1 protein, N-His, Primary: Anti-ADGRE1 (orb156726) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 95 kDa, Observed band size: 32 kDa.