EED Antibody

• Catalog Number: orb666552
• Category: Antibodies
• Description: The EED Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide of human EED. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide of human EED. The exact sequence is proprietary.
• UniProt ID: O75530, Q921E6
• Tested applications: IF, IHC, WB
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Polycomb protein EED; hEED; WD protein associating
• Research Area: Epigenetics, Infectious Diseases
• Note: For research use only
• Entrez: 13626, 8726

Western blot analysis of EED expression in MCF7 (A), A549 (B), mouse lung (C) whole cell lysates. (Predicted band size: 45; 50; 53 kD; Observed band size: 50-75 kD)

Immunofluorescent analysis of EED staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.