(Mouse) Eed Antibody (Center)

• Catalog Number: orb1926542
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This mouse Eed antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 269-303 amino acids from the Central region of mouse Eed.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q921E6
• MW: 50198 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:25
• Clone Number: RB51180
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Polycomb protein EED, Eed
• Note: For research use only

All lanes: Anti-Eed Antibody (Center) at 1:1000 dilution. Lane 1: A431 whole cell lysates. Lane 2: NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining (Mouse) Eed in Mouse spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.