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Anti-DDB1 Antibody

Catalog Number: orb340855

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb340855
CategoryAntibodies
DescriptionRabbit polyclonal antibody to DDB1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide of human DDB1
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1:1000, IHC-P: 1:50-1:100
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetDDB1
Entrez13194, 1642
UniProt IDQ9ESW0, Q16531, Q3U1J4
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesXAP1; DNA damage-binding protein 1; DDB p127 subun
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-DDB1 Antibody

Western blot analysis of DDB1 expression in A549 (A), mouse brain (B), mouse spleen (C), mouse liver (D) whole cell lysates. (Predicted band size: 50; 126 kD; Observed band size: 127 kD)

Anti-DDB1 Antibody

Immunohistochemical analysis of DDB1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-DDB1 Antibody

Immunofluorescent analysis of DDB1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • Anti-DDB1 Antibody [orb312104]

    FC,  ICC,  IF,  IHC,  IHC-Fr,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Goat anti-DDB1 Antibody [orb18292]

    ELISA,  IHC,  WB

    Bovine, Canine, Human, Mouse, Rat

    Goat

    Polyclonal

    Unconjugated

    100 μg
  • Rabbit anti-DDB1 Antibody [orb1530162]

    IHC,  IP,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    2 μg
  • Rabbit anti-DDB1 Antibody [orb1530163]

    IHC,  IP,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    20 μg
  • DDB1 Antibody [orb415085]

    ELISA,  IF,  IHC

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 50 μg