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WDR26 Rabbit Polyclonal Antibody

Catalog Number: orb11572

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb11572
CategoryAntibodies
DescriptionWDR26 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine, Gallus
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human WDR26 (101-200/514aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1µg/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW72 kDa
TargetWDR26
UniProt IDQ9H7D7
RRIDAB_10750055
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesmyocardial ischemic preconditioning upregulated pr
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NoteFor research use only
Expiration Date12 months from date of receipt.
WDR26 Rabbit Polyclonal Antibody

Blank control: RSC96 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA (green).

WDR26 Rabbit Polyclonal Antibody

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-WDR26 antibody (orb11572), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

WDR26 Rabbit Polyclonal Antibody

Sample: Placenta (Mouse) Lysate at 40 ug, Primary: Anti-WDR26 (orb11572) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 72 kD, Observed band size: 70 kD.

WDR26 Rabbit Polyclonal Antibody

Tissue/Cell: human laryngocarcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-WDR26 Polyclonal Antibody, Unconjugated (orb11572) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

WDR26 Rabbit Polyclonal Antibody

Tissue/Cell: MCF7, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (WDR26) Polyclonal Antibody, Unconjugated (orb11572) 1:200, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

WDR26 Rabbit Polyclonal Antibody

Tissue/Cell: rat heart tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-WDR26 Polyclonal Antibody, Unconjugated (orb11572) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

  • Anti-WDR26 Antibody [orb668641]

    WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 50 μl
  • WDR26 Rabbit Polyclonal Antibody [orb588536]

    WB

    Human

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • WDR26 Rabbit Polyclonal Antibody [orb585378]

    WB

    Bovine, Canine, Equine, Guinea pig, Human, Rabbit, Rat, Zebrafish

    Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • WDR26 Rabbit Polyclonal Antibody (HRP) [orb2082035]

    WB

    Human

    Rabbit

    Polyclonal

    HRP

    100 μl
  • WDR26 Rabbit Polyclonal Antibody (FITC) [orb2082036]

    WB

    Human

    Rabbit

    Polyclonal

    FITC

    100 μl