Anti-CXCR4 antibody(DMC680), IgG1 Chimeric mAb

• Catalog Number: orb1826957
• Category: Antibodies
• Description: Anti-CXCR4 antibody(DMC680); IgG1 Chimeric mAb
• Target: CXCR4
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit/Human Fc chimeric IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• UniProt ID: P61073, A0A0U3FJG0, A0A0U3GXA9
• Tested applications: FC
• Dilution range: Flow Cyt 1:100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: DMC680
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD184; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; L
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

CXCR4 protein is expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-CXCR4 on Expi293 cells transfected with human CXCR4 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

Flow cytometry analysis of antigen binding of anti-human CXCR4 mAb (orb1826957). (A) orb1826957 does not bind to hepG2 cells that do not express CXCR4. (B) A clear peak shift of orb1826957 was seen compared to the control when incubated with CXCR4-expressing Hela cells, indicating strong binding of orb1826957 to CXCR4. Antibodies were incubated at 5 μg/mL.