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Catalog Number | orb10305 |
---|---|
Category | Antibodies |
Description | CXCR4 Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Bovine, Rabbit |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from the middle of human CXCR4 (1-38/352aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 40 kDa |
Target | CXCR4 |
UniProt ID | P61073 |
RRID | AB_10753279 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; S Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blank control: mouse splenocytes (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µl in 100 µL 1X PBS containing 0.5% BSA (green).
Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CXCR4 antibody (orb10305), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10305, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.
Sample: Brain (Rat) Lysate at 30 ug, Heart (Rat) Lysate at 30 ug, Primary: Anti-CXCR4 (orb10305) at 1/200 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/3000 dilution, Predicted band size: 39 kD, Observed band size: 39 kD.
Sample: Lane 1: Blood cell (Mouse) Lysate at 40 ug, Lane 2: Lymph node (Mouse) Lysate at 40 ug, Lane 3: Bone (Rat) Lysate at 40 ug, Lane 4: Spleen (Rat) Lysate at 40 ug, Primary: Anti-CXCR4 at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 44 kD.
Sample: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-CXCR4 (orb10305) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kD, Observed band size: 40 kD.
Tissue/cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-CD184/CXCR4 Polyclonal Antibody, Unconjugated (orb10305) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
FC, IHC, WB | |
Equine, Porcine, Rabbit | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Monkey, Mouse, Rat | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Porcine, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Porcine, Primate, Rabbit, Rat, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |