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CXCR4 Rabbit Polyclonal Antibody

Catalog Number: orb10305

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10305
CategoryAntibodies
DescriptionCXCR4 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Rabbit
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from the middle of human CXCR4 (1-38/352aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW40 kDa
TargetCXCR4
UniProt IDP61073
RRIDAB_10753279
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesC-X-C chemokine receptor type 4; CXC-R4; CXCR-4; S
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NoteFor research use only
Expiration Date12 months from date of receipt.
CXCR4 Rabbit Polyclonal Antibody

Blank control: mouse splenocytes (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µl in 100 µL 1X PBS containing 0.5% BSA (green).

CXCR4 Rabbit Polyclonal Antibody

Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CXCR4 antibody (orb10305), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10305, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

CXCR4 Rabbit Polyclonal Antibody

Sample: Brain (Rat) Lysate at 30 ug, Heart (Rat) Lysate at 30 ug, Primary: Anti-CXCR4 (orb10305) at 1/200 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/3000 dilution, Predicted band size: 39 kD, Observed band size: 39 kD.

CXCR4 Rabbit Polyclonal Antibody

Sample: Lane 1: Blood cell (Mouse) Lysate at 40 ug, Lane 2: Lymph node (Mouse) Lysate at 40 ug, Lane 3: Bone (Rat) Lysate at 40 ug, Lane 4: Spleen (Rat) Lysate at 40 ug, Primary: Anti-CXCR4 at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 44 kD.

CXCR4 Rabbit Polyclonal Antibody

Sample: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-CXCR4 (orb10305) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kD, Observed band size: 40 kD.

CXCR4 Rabbit Polyclonal Antibody

Tissue/cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-CD184/CXCR4 Polyclonal Antibody, Unconjugated (orb10305) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

  • CXCR4 Rabbit Polyclonal Antibody [orb573686]

    FC,  IHC,  WB

    Equine, Porcine, Rabbit

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • LAP3 Rabbit Polyclonal Antibody [orb583305]

    WB

    Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • Fusin (phospho Ser339) rabbit pAb [orb770434]

    ELISA,  IF,  IHC-P,  WB

    Human, Monkey, Mouse, Rat

    Polyclonal

    Unconjugated

    50 μl, 100 μl
  • Anti-CD184 (Phospho-S339) Antibody [orb214761]

    IF,  IH,  WB

    Human, Mouse, Porcine, Primate, Rat

    Rabbit

    Polyclonal

    Unconjugated

    30 μl, 100 μl, 200 μl
  • Anti-CD184 Antibody [orb304731]

    IF,  IH,  WB

    Human, Mouse, Porcine, Primate, Rabbit, Rat, Sheep

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 30 μl