Anti-CUX1 Antibody

• Catalog Number: orb666407
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CUX1
• Target: CUX1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant fusion protein of human CUX1. The exact sequence is proprietary.
• UniProt ID: P53564, P70403, P39880, Q13948
• Tested applications: IF, WB
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CUX1 antibody, anti-CUTL1 antibody, anti-Home
• Note: For research use only
• Entrez: 13047, 1523

Western blot analysis of CUX1 expression in MCF7 (A), mouse brain (B), mouse testis (C), rat kidney (D) whole cell lysates. (Predicted band size: 72-77; 147-165 kD; Observed band size: 200; 77 kD)

Immunofluorescent analysis of CUX1 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.