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Anti-CUX1 Antibody

Catalog Number: orb213810

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb213810
CategoryAntibodies
DescriptionRabbit polyclonal antibody to CUX1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CUX1. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetCUX1
Entrez13047, 1523
UniProt IDP53564, P70403, P39880, Q13948
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti CUX1 antibody, anti CUTL1 antibody, anti Home
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-CUX1 Antibody

Western blot analysis of CUX1 expression in mouse muscle (A), rat muscle (B) whole cell lysates. (Predicted band size: 72-77; 147-165 kD; Observed band size: 180 kD)

Anti-CUX1 Antibody

Immunohistochemical analysis of CUX1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-CUX1 Antibody

Immunofluorescent analysis of CUX1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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