CUX1 Antibody (C-term)

• Catalog Number: orb1930297
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB23382
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IF: 1:25, WB: 1:2000, WB: 1:2000, IHC-P: 1:25, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 164187 Da
• Target: This CUX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1347-1374 amino acids of human CUX1.
• UniProt ID: P39880
• NCBI: NP_001189474.1, NP_001189472.1, NP_853530.2, NP_852477.1, NP_001189473.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Homeobox protein cut-like 1, CCAAT displacement pr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling CUX1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).

Staining CUX1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining CUX1 in human lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes: Anti-CUX1 Antibody (C-term) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 164 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CUX1 Antibody (C-term) at 1:2000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: MOLT-4 whole cell lysate. Lane 3: SH-SY5Y whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 164 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CUX1 Antibody (C-term) at 1:1000 dilution. Lane 1: SH-SY5Y whole cell lysate. Lane 2: SK-BR-3 whole cell lysate. Lane 3: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size: 200 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.