CRMP-5 Mouse Monoclonal Antibody

SKU: orb1473693

Description

The CRMP-5 Antibody is suitable for IF, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human CRMP-5. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column.

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB (1/1000 - 1/2000), IF/IC (1/10 - 1/50)
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Immunogen: Recombinant fusion protein of human CRMP-5. The exact sequence is proprietary.
• Target: DPYSL5
• Purification: This antibody is purified through a protein G column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CRMP5; ULIP6; Dihydropyrimidinase-related protein 5; DRP-5; CRMP3-associated molecule; CRAM; Collapsin response mediator protein 5; CRMP-5; UNC33-like phosphoprotein 6; ULIP-6

Western blot analysis of CRMP-5 expression in SHSY5Y (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 61 kD; Observed band size: 61 kD)

Immunofluorescent analysis of CRMP-5 staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

DPYSL5

UniProt

Q9EQF6, Q9JHU0, Q9BPU6

NCBI Gene ID

100910964, 65254, 65208, 56896