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Description
Research Area
Images & Validation
−| Tested Applications | IF, WB |
|---|---|
| Dilution Range | IF: 1:25, WB: 1:4000 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,Κ |
| Immunogen | This DPYSL5 antibody is generated from a mouse immunized with a recombinant protein of human DPYSL5. Antigen Region: 300-584 aa. |
| Target | DPYSL5 |
| Molecular Weight | 61421 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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All lanes: Anti-DPYL5 Antibody at 1:4000 dilution. Lane 1: SH-SY5Y whole cell lysate. Lane 2: mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on SH-SY5Y cell line. The nuclear counter stain is DAPI (blue).
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Protocol Information
DPYSL5 Antibody (orb1787900)
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