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DPYSL5 Antibody
Description
Images & Validation
−| Tested Applications | IF, WB |
|---|---|
| Dilution range | IF - 1:25, WB - 1:4000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1,k |
| Molecular Weight | 61421 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Disclaimer | For research use only |
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All lanes: Anti-DPYL5 Antibody at 1:4000 dilution. Lane 1: SH-SY5Y whole cell lysates. Lane 2: mouse brain lysates. Lane 3: rat brain lysates. Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line) cells labeling DPYL5 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on SH-SY5Y cell line. The nuclear counter stain is DAPI (blue).
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DPYSL5 Antibody (orb1926252)
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