DPYSL5 Antibody

• Catalog Number: orb1926252
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Target: This DPYL5 antibody is generated from a mouse immunized with a recombinant protein of human DPYL5.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1,k
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• UniProt ID: Q9BPU6
• MW: 61421 Da
• Tested applications: IF, WB
• Dilution range: IF: 1:25, WB: 1:4000
• Clone Number: 1503CT789.209.250.52
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Dihydropyrimidinase-related protein 5, DRP-5, CRMP
• Research Area: Neuroscience
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-DPYL5 Antibody at 1:4000 dilution. Lane 1: SH-SY5Y whole cell lysates. Lane 2: mouse brain lysates. Lane 3: rat brain lysates. Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line) cells labeling DPYL5 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on SH-SY5Y cell line. The nuclear counter stain is DAPI (blue).