CREB (Ser133) Antibody

SKU: orb319490

Description

Our Anti-CREB (Ser133) rabbit polyclonal phosphospecific primary antibody from Biorbyt is produced in-house. It detects rat CREB (Ser133) and is antigen affinity purified from pooled serum.

Research Area

Epigenetics & Chromatin, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB: 0.736111111, IHC: 1:100-1:1000
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Human, Primate, Sheep, Xenopus, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 of rat CREB, conjugated to keyhole limpet hemocyanin (KLH).
• Target: CREB (Ser133)
• Molecular Weight: 45
• Purification: Antigen Affinity Purified from Pooled Serum
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol.
• Concentration: Lot Dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Active transcription factor CREB antibody, cAMP response element binding protein 1 antibody, cAMP response element binding protein antibody, cAMP responsive element binding protein 1 antibody, cAMP-responsive element-binding protein 1 antibody, CREB antibody, CREB-1 antibody, CREB1 antibody, CREB1_HUMAN antibody, Cyclic AMP-responsive element-binding protein 1 antibody, MGC9284 antibody, OTTHUMP00000163864 antibody, OTTHUMP00000163865 antibody, OTTHUMP00000206660 antibody, OTTHUMP00000206662 antibody, , TTHUMP00000206667 antibody, Transactivator protein antibody

Immunolabeling of a section of mouse piriform cortex labeled with Anti-Phospho-Ser133 CREB (Cat. orb319490, red, 1:1000). Cell nuclei are visualized with DAPI DNA stain (blue).

Immunolabeling of mouse cerebral cortex (top) and habenula (bottom) brain sections labeled with anti-phospho-ser133 CREB (Cat. orb319490, DAB, 1:100).

Immunolabeling of mouse hippocampal section labeled with anti-phospho-ser133 CREB (Cat. orb319490, DAB, 1:100, top). The image on the bottom is a magnification of the dentate gyrus section of the hippocampus.

Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).

Quick Database Links

Gene Symbol

CREB (Ser133)

UniProt

P15337