Phospho-CREB (Ser133) (4D11) rabbit mAb Antibody

• Catalog Number: orb1946255
• Category: Antibodies
• Description: Phospho-CREB (Ser133) (4D11) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: CREBS133-4D11
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser133 of human phospho CREB
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P16220
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Cyclic AMP-responsive element-binding protein 1, C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of SK-N-MC cells untreated (red) or treated with forskolin (green) using Phospho-CREB (Ser133) (4D11) Rabbit mAb CREBS133-4D11 at 0.01 ug/mL #orb1946255, or concentration-matched Rabbit (G9) mAb IgG Isotype Control #orb1946241 for cells untreated (black) or treated with forskoin (blue).

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or untreated (red) or treated with PDGF (green) using Phospho-CREB (Ser133) antibody CREBS133-4D11 at 0.1μg/mL. Cat #orb1946255.

Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with calyculin A (green) using 1ng/mL Phospho-CREB (Ser133) antibody CREBS133-4D11. Cat. #orb1946255.

Western blot analysis of SK.N.MC cell extract untreated or treated with forskolin and IBMX using 0.1μg/mL Phospho-CREB (Ser133) antibody CREBS133-4D11. Cat. #orb1946255.

Flow cytometric analysis of SK.N.MC cells secondary antibody only negative control (blue) or untreated (red) or treated with forskolin (green) using 0.01 ug/mL of Phospho-CREB (Ser133) antibody CREBS133-4D11 (Cat. #orb1946255) or Company C antibody at 0.02 ug/mL (manufacturer's recommended concentration).