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Anti-Collagen 1 alpha 1 Antibody

Catalog Number: orb1289711

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb1289711
CategoryAntibodies
DescriptionRabbit Polyclonal antibody to Collagen 1 alpha 1
TargetCOL1A1
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence of human Collagen 1 alpha 1. The exact sequence is proprietary.
UniProt IDP02454, P11087, P02452
Tested applicationsIF, IH, IP, WB
Dilution rangeWB: 1:500-1:1000, IH: 1:50-1:200, IF:IC: 1:50-1:200, IP: 1:50-1:100
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesCollagen alpha-1(I) chain; Alpha-1 type I collagen
NoteFor research use only
Entrez1277, 29393, 12842
Anti-Collagen 1 alpha 1 Antibody

Western blot analysis of Collagen 1 alpha 1 expression in mouse spleen (A), rat lung (B) whole cell lysates. (Predicted band size: 138 kD; Observed band size: 138 kD)

Anti-Collagen 1 alpha 1 Antibody

Immunohistochemical analysis of Collagen 1 alpha 1 staining in human lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Collagen 1 alpha 1 Antibody

Immunofluorescent analysis of Collagen 1 alpha 1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

  • Collagen Type I Antibody [orb345343]

    DOT,  ELISA,  FLISA,  IHC,  IP,  WB

    Bovine, Human, Mouse, Porcine, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
  • Collagen Type I Antibody [orb345344]

    DOT,  ELISA,  FLISA,  IHC,  IP,  WB

    Bovine, Human, Mouse, Porcine, Rat

    Rabbit

    Polyclonal

    Unconjugated

    500 μg
  • Collagen Type IV Antibody [orb345352]

    DOT,  ELISA,  IHC,  IP,  WB

    Bovine, Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
  • Collagen Type IV Antibody [orb345353]

    DOT,  ELISA,  IHC,  IP,  WB

    Bovine, Human

    Rabbit

    Polyclonal

    Unconjugated

    500 μg
  • Collagen X antibody [orb10444]

    ELISA,  IHC-P,  WB

    Bovine, Canine, Gallus, Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg