Collagen X antibody

• Catalog Number: orb10444
• Category: Antibodies
• Description: Collagen X antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Bovine, Canine, Gallus, Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Collagen X. Please contact us for the exact immunogen sequence. The peptide is available as orb374717.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: IHC-P: 1:300-500, WB: 1:200-2000
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 66 kDa
• Target: Collagen X
• Entrez: 1300
• UniProt ID: Q03692, Q05306
• NCBI: 000484, 000493
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Alternative names: anti-Col10a 1 antibody, anti-Collagen alpha 1(X) c
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Fan, Litong et al. Enhancement of the Chondrogenic Differentiation of Mesenchymal Stem Cells and Cartilage Repair by Ghrelin J. Orthop. Res., (2019)

PubMed: 30644571

Applications

IHC

Reactivity

Rat

Schreiber, Caroline et al. Loss of ASAP1 in mice impairs adipogenic and osteogenic differentiation of mesenchymal progenitor cells through dysregulation of FAK/Src and AKT signaling PLoS Genet., 15, e1008216 (2019)

PubMed: 31246957

Applications

IHC

Reactivity

Mouse

IHC-P staining of rat liver tissue using Collagen X antibody (2.5 ug/ml)

Immunohistochemical staining of rat liver tissue using Collagen X antibody (2.5 ug/ml)

IHC-P staining of rat liver tissue using anti-Collagen X (2.5 ug/ml)

Western blot analysis of mouse pancreas (lane 1), human breast cancer (lane 2), human mammary fibroma (lane 3) using Collagen X antibody (1 ug/ml)

WB analysis of mouse spinal cord (lane 1), mouse liver (lane 2), mouse pancreas (lane 3), human breast cancer (lane 4), human mammary fibroma (lane 5) using Collagen X antibody (1 ug/ml)

Western blot analysis of mouse spinal cord (lane 1), mouse liver (lane 2), mouse pancreas (lane 3), human breast cancer (lane 4), human mammary fibroma (lane 5) using Collagen X antibody (1 ug/ml)

WB analysis of mouse pancreas (lane 1), human breast cancer (lane 2), human mammary fibroma (lane 3) using Collagen X antibody (1 ug/ml)

Immunohistochemical staining of paraffin embedded mouse bone marrow tissue using anti-Collagen X (2.5 ug/ml)