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Collagen 4 alpha 1 antibody
Description
Images & Validation
−| Tested Applications | ICC, IF, IHC-P, WB |
|---|---|
| Dilution range | IF/ICC: 1:100-600, IHC-P: 1:100-600, WB: 1:200-1000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Collagen 4 alpha 1. Please contact us for the exact immunogen sequence. The peptide is available as orb13012. |
| Target | Collagen 4 alpha 1 |
| Molecular Weight | 161 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Disclaimer | For research use only |
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WB analysis of mouse brain (lane 1), mouse spinal cord (lane 2), mouse colon (lane 3), rat bladder (lane 4), rat ovary (lane 5), U251 cells (lane 6) using Collagen 4 alpha 1 antibody (1 ug/ml)

Immunofluorescence image of rat stomach tissue using anti-Collagen 4 alpha 1 (2.5 ug/ml)

IHC-P image of rat stomach tissue using anti-Collagen 4 alpha 1 (2.5 ug/ml)

Immunohistochemical staining of rat skin tissue using Collagen 4 alpha 1 antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat stomach tissue using anti-Collagen 4 alpha 1 (2.5 ug/ml)

Immunohistochemical staining of rat stomach tissue using anti-Collagen 4 alpha 1 (2.5 ug/ml)

IHC-P staining of rat skin tissue using Collagen 4 alpha 1 antibody (2.5 ug/ml)

IF analysis of rat stomach tissue using Collagen 4 alpha 1 antibody (2.5 ug/ml)

IHC-P image of rat stomach tissue using Collagen 4 alpha 1 antibody (2.5 ug/ml)

Western blot analysis of mouse brain (lane 1), mouse spinal cord (lane 2), mouse colon (lane 3), rat bladder (lane 4), rat ovary (lane 5), U251 cells (lane 6) using Collagen 4 alpha 1 antibody (1 ug/ml)

Immunohistochemical staining of paraffin embedded rat spinal cord tissue using anti-Collagen 4 alpha 1 (2.5 ug/ml)
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Collagen 4 alpha 1 antibody (orb382133)
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