Clathrin Heavy Chain Antibody

SKU: orb43680

Description

Mouse Monoclonal to Clathrin (H chain).

Research Area

Autophagic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, FC, ICC, IP, WB
• Reactivity: Bovine, Human, Mouse, Porcine, Rat
• Application Notes:
  Flow cytometry: Recommended dilution: 4-10 μg/ml. Intracellular staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgM
• Clone No.: BF-06
• Target: Clathrin Heavy Chain
• Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CHC, CLHC, MRD56

Immunocytochemistry staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Immunocytochemistry staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Separation of MCF-7 cells stained using anti-Clathrin (BF-06) purified antibody (concentration in sample 2 µg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Quick Database Links

Gene Symbol

Clathrin Heavy Chain

UniProt

Q00610

NCBI Gene ID

1213