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Item 1 of 3
Anti-Clathrin Heavy Chain Purified
Catalog Number: orb43680
| Catalog Number | orb43680 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to Clathrin (H chain). |
| Clonality | Monoclonal |
| Clone Number | BF-06 |
| Tested applications | ELISA, FC, ICC, IP, WB |
| Reactivity | Bovine, Human, Mouse, Porcine, Rat |
| Isotype | Mouse IgM |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| Conjugation | Unconjugated |
| Target | Clathrin Heavy Chain |
| Entrez | 1213 |
| UniProt ID | Q00610 |
| RRID | AB_10989313 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Alternative names | Anti-Clathrin heavy chain antibody Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: Recommended dilution: 4-10 μg/ml. Intracellular staining. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Immunocytochemistry staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Separation of MCF-7 cells stained using anti-Clathrin (BF-06) purified antibody (concentration in sample 2 µg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
