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Catalog Number | orb1930292 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB24434 |
Tested applications | FC, IHC-P, WB |
Predicted Reactivity | Mouse, Rat |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 191615 Da |
Target | This CLTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1019-1048 amino acids from the Central region of human CLTC. |
UniProt ID | Q00610 |
NCBI | NP_004850.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Clathrin heavy chain 1, Clathrin heavy chain on ch Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
CLTC Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CLTC antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLTC antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Anti-CLTC Antibody (Center) at 1:1000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 192 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.