Clathrin heavy chain Rabbit Polyclonal Antibody

• Catalog Number: orb100509
• Category: Antibodies
• Description: Clathrin heavy chain Rabbit Polyclonal Antibody
• Target: CLTC
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Frog, Insect, Invertebrate, Mouse, Porcine, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human Clathrin heavy chain. (401-500/1675aa)
• UniProt ID: Q00610
• MW: 192 kDa
• Tested applications: ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CHC17; Clathrin heavy chain 1; Clathrin heavy chai
• Research Area: Cell Biology, Membrane Transport
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Clathrin heavy chain) polyclonal Antibody, Unconjugated (orb100509) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Human SH-SY5Y cell lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human U-2 OS cell lysates, Primary: Anti-Clathrin heavy chain (orb100509) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 192 kDa, Observed band size: 182 kDa.

Sample: Olfactory bulb (Mouse) Lysate at 40 ug, Primary: Anti-Clathrin heavy chain (orb100509) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 192 kD, Observed band size: 192 kD.