Clathrin heavy chain Rabbit Polyclonal Antibody

SKU: orb100509

Description

Clathrin heavy chain Rabbit Polyclonal Antibody

Research Area

Cell Biology, Membrane Transport

Images & Validation

• Tested Applications: ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:50-200
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Frog, Insect, Invertebrate, Mouse, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Clathrin heavy chain. (401-500/1675aa)
• Target: CLTC
• Molecular Weight: 192 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CHC17; Clathrin heavy chain 1; Clathrin heavy chain 2; Clathrin heavy chain; Clathrin heavy chain like 1; Clathrin heavy chain on chromosome 17; Clathrin heavy polypeptide; CLH 17; CLH 22; CLH-17; CLH1_HUMAN; CLH17; CLH22; CLTC; CLTCL; CLTCL1; CLTCL2; CLTD; Hc; KIAA0034.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Clathrin heavy chain) polyclonal Antibody, Unconjugated (orb100509) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Human SH-SY5Y cell lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human U-2 OS cell lysates, Primary: Anti-Clathrin heavy chain (orb100509) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 192 kDa, Observed band size: 182 kDa.

Sample: Olfactory bulb (Mouse) Lysate at 40 ug, Primary: Anti-Clathrin heavy chain (orb100509) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 192 kD, Observed band size: 192 kD.

Quick Database Links

Gene Symbol

CLTC

UniProt

Q00610