Anti-CD52 [Alemtuzumab (Campath-1H)]

SKU: orb348891

Description

Rabbit monoclonal antibody to CD52

Images & Validation

• Tested Applications: ELISA, FC, IHC-Fr, IHC-P, WB
• Reactivity: Human, Primate

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: Human IgG
• Clone No.: Alemtuzumab (Campath-1H)
• Immunogen: Human lymphocytes.
• Target: CD52
• Purity: Purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

CDW52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5; YTH66.9HL

Flow-cytometry using anti-CD52 antibody Campath-1H Cynomolgus monkey lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Campath-1H (orb411594, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Flow-cytometry using anti-CD52 antibody Campath-1H Rhesus monkey lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Campath-1H (panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Quick Database Links

Gene Symbol

CD52

UniProt

P31358

References

Riechmann, L. et al. Reshaping human antibodies for therapy Nature, 332, 323-327 (1988)

PubMed: 3127726