Anti-CD52 [Alemtuzumab (Campath-1H)]

• Catalog Number: orb1152527
• Category: Antibodies
• Description: Rat monoclonal antibody to CD52
• Target: CD52
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: Human IgG2b
• Conjugation: Unconjugated
• Reactivity: Human, Primate
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: Human lymphocytes.
• UniProt ID: P31358
• Tested applications: ELISA, FC, IHC-Fr, IHC-P, WB
• Clone Number: Alemtuzumab (Campath-1H)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CDW52; Cambridge pathology 1 antigen; Epididymal s
• Research Area: Pharmacology & Drug Discovery
• Note: For research use only

Flow-cytometry using anti-CD52 antibody Campath-1H Cynomolgus monkey lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Campath-1H (orb411594, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Flow-cytometry using anti-CD52 antibody Campath-1H Rhesus monkey lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Campath-1H (panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed rat thymus tissue with anti-CD52 antibody Campath-1H. Immunofluorescence analysis of fixed rat thymus tissue stained with the chimeric human IgG1 version of Campath-1H (orb348863) at 5 µg/ml followed by a FITC conjugated secondary antibody, showing membrane and cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right; orb348863 (FITC), DAPI and merged channels.

Immunofluorescent staining of rat thymus using anti-CD52 antibody. Formaldehyde-fixed rat thymus slices were stained with orb348863 at 5 µg/ml and detected with a FITC-conjugated secondary antibody.

Immunohistochemical staining of human thyroid cancer using anti-CD52 antibody. Formalin fixed human thyroid cancer slices were were stained with a orb348863 at 5 µg/ml.

Immunohistochemical staining of rat epididymis tissue using anti-CD52 antibody Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat epididymis tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (orb348863) was used to stain samples at a concentration of 5 µg/ml.

Immunohistochemical staining of rat spleen tissue using anti-CD52 antibody Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (orb348863) was used to stain samples at a concentration of 5 µg/ml.

Immunohistochemical staining of rat thymus tissue using anti-CD52 antibody Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat thymus tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (orb348863) was used to stain samples at a concentration of 5 µg/ml.