Cart summary

You have no items in your shopping cart.

Anti-CD45 Antibody

Catalog Number: orb665854

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb665854
CategoryAntibodies
DescriptionRabbit polyclonal antibody to CD45
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD45. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1000, IHC-P: 1-50-200, IF: 1-100-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetPTPRC
Entrez5788
UniProt IDP06800, P08575
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesAnti-CD45 antibody, anti-Receptor-type tyrosine-pr
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-CD45 Antibody

Western blot analysis of CD45 expression in K562 (A), SHSY5Y (B) whole cell lysates. (Predicted band size: 147 kD; Observed band size: 180 kD)

Anti-CD45 Antibody

Immunohistochemical analysis of CD45 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-CD45 Antibody

Immunofluorescent analysis of CD45 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

  • CD45 Monoclonal Antibody [orb688878]

    ELISA,  FC,  IF,  IHC,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    50 μl, 100 μl
  • Rabbit anti-CD45 Recombinant Monoclonal Antibody [orb1519973]

    FC,  ICC,  IHC,  IP,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    10 μl
  • Rabbit anti-CD45 Recombinant Monoclonal Antibody [orb1519975]

    FC,  ICC,  IHC,  IP,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    100 μl
  • Anti-Hu CD45 Purified [orb44266]

    FC,  ICC,  IHC-P,  IP,  WB

    Human

    Monoclonal

    Unconjugated

    0.1 mg
  • Anti-CD45/PTPRC Antibody [orb196266]

    FC,  IF,  IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg