Anti-CD40 antibody(DM100), Rabbit mAb

• Catalog Number: orb1174068
• Category: Antibodies
• Description: Anti-CD40 antibody(DM100), Rabbit mAb
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: DM100
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Recombinant Antibody
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD40
• UniProt ID: P25942
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD40; Bp50; CDW40; MGC9013; TNFRSF5; p50
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD40 protein, mFc-His tagged protein orb689376 can bind Rabbit anti-CD40 monoclonal antibody (clone: DM100) in a linear range of 0.64-80 ng/ml.

A. Flow cytometry analysis with Anti-CD40 (DM100) on Expi293 cells transfected with human CD40 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Flow cytometry analysis of antigen binding of rabbit anti-human CD40 mAb (orb1174068). (A) orb1174068 does not bind to Jurkat cells that do not express CD40. (B) A clear peak shift of orb1174068 was seen compared to the control when incubated with CD40-expressing Raji cells, indicating strong binding of orb1174068 to CD40. Antibodies were incubated at 2 μg/mL.

Anti-CD40 antibody (SKU# orb1174068) at 1/1000 dilution.Lane: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 30 kDa.Observed band size: 45 kDa

A. orb1174068 at 10 μg/ml staining CD40 in Raji cells by IHC (SKU# orb1174068); B. orb1174068 at 10 μg/ml staining CD40 in human tonsil tissue by IHC (SKU# orb1174068)