Anti-CD21 antibody(DMC280); IgG1 Chimeric mAb

• Catalog Number: orb1471673
• Category: Antibodies
• Description: Anti-CD21 antibody(DMC280), IgG1 Chimeric mAb
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: DMC280
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Rabbit/Human Fc chimeric IgG1
• Antibody Type: Recombinant Antibody
• Dilution range: Flow Cyt 1:100; WB 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD21
• UniProt ID: P20023
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: C3DR; CD21; CR; CVID7; SLEB9
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis with Anti-CD21 on Expi293 cells transfected with human CD21 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

Anti-CD21 antibody (SKU# orb1471673) at 1/1000 dilution.Lane: RAJI, whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 113 kDa.Observed band size: 150 kDa

Flow cytometry analysis of antigen binding of anti-human CD21 mAb (orb1471673). (A) orb1471673 does not bind to Jurkat cells that do not express CD21. (B) A clear peak shift of orb1471673 was seen compared to the control when incubated with CD21-expressing Raji cells, indicating strong binding of orb1471673 to CD21. Antibodies were incubated at 10 μg/mL.