CR2 Rabbit Polyclonal Antibody

• Catalog Number: orb7143
• Category: Antibodies
• Description: CR2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CR2 (981-1092/1092aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 111 kDa
• Target: CR2
• UniProt ID: P20023
• RRID: AB_10934575
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: C3DR; CD 21; CD21; CD21 antigen; Complement C3d re
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): Molt-4 Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD21/AF647 Conjugated antibody (orb7143/AF647), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions.

Blank control (blue): U-87MG Cells (fixed with 2% paraformaldehyde (10 min). Primary Antibody: Rabbit Anti-CD21/AF647 Conjugated antibody (orb7143/AF647), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions.

Blank control: Raji cells (blue). Primary Antibody: Rabbit Anti-CD21 antibody (orb7143), dilution: 1 µg in 100 1 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Raji (Human) Cell Lysate at 30 ug, Primary: Anti-CD21 (orb7143) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 111 kD, Observed band size: 111 kD.