Anti-Complement Receptor 1 & 2 [7G6]

• Catalog Number: orb348930
• Category: Antibodies
• Description: Rat monoclonal antibody to Complement Receptor 1 & 2
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 7G6
• Tested applications: Blocking, FC, IHC-Fr, IP, WB
• Reactivity: Mouse
• Isotype: IgG2b
• Immunogen: Purified Mouse CR1.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Complement Receptor 1 & 2
• UniProt ID: P17927, P19070
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD21 & CD35
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Kinoshita, T. et al. Monoclonal antibodies to mouse complement receptor type 1 (CR1). Their use in a distribution study showing that mouse erythrocytes and platelets are CR1-negative J Immunol, 140, 3066-3072 (1988)

PubMed: 2966205

Flow-cytometry using anti-complement receptors 1 & 2 antibody 7G6 Mouse lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of 7G6 (orb348914, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Immunohistochemical staining of rat stomach using anti-Complement receptors 1&2 antibody orb348914 Formalin fixed rat stomach slices were were stained with orb348914 at 3 µg/ml.