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Description
Images & Validation
−| Tested Applications | IF, WB |
|---|---|
| Dilution range | WB: 1-500-2000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic peptide of human CCR7. The exact sequence is proprietary. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−CCR7 Rabbit Polyclonal Antibody [orb10276]
FC, ICC, IF, IHC-Fr, IHC-P, WB
Canine
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl, 200 μlCCR7 Antibody (N-term) [orb1930722]
FC, IF, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μlHuman Chemokine C-C-Motif Receptor 7 (CCR7) ELISA Kit [orb775999]
Human
0.16-10 ng/mL
0.06 ng/mL
48 T, 96 TMouse Chemokine C-C-Motif Receptor 7 (CCR7) ELISA Kit [orb777525]
Mouse
0.16-10 ng/mL
0.056 ng/mL
48 T, 96 T

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Western blot analysis of CCR7 expression in Jurkat (A) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 45 kD)

Immunofluorescent analysis of CCR7 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Quick Database Links
UniProt Details
−NCBI Gene Details
−Documents Download
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Protocol Information
CCR7 Antibody (orb666219)
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