CCR7 Antibody (N-term)

• Catalog Number: orb1930722
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB21736
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:500, WB: 1:1000, WB: 1:500-1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 42874 Da
• Target: This CCR7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-37 amino acids from the N-terminal region of human CCR7.
• UniProt ID: P32248
• NCBI: NP_001829.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: C-C chemokine receptor type 7, C-C CKR-7, CC-CKR-7
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

CCR7 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of CCR7 Antibody (N-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

CCR7 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CCR7 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

CCR7 Antibody (N-term) western blot analysis in 293 (lane 1), Ramos (lane 2), MDA-MB231 (lane 3) cell line and mouse spleen tissue (lane 4) lysates (35 ug/lane). This demonstrates the CCR7 antibody detected the CCR7 protein (arrow).

Anti-CCR7 Antibody (Nterm) at 1:500 dilution + MOLT-4 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CCR7 Antibody (N-term) at 1:500-1:1000 dilution. Lane 1: MDA-MB-231 whole cell lysate. Lane 2: MOLT-4 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.