c-Myc tag Antibody

SKU: orb43629

Description

Mouse Monoclonal to c-Myc.

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB
• Reactivity: All, Human
• Application Notes:
  Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive tissue: perfused brain sections, liver, spleen. Immunoprecipitation: Recommended dilution: 1-5 μg/ml; this antibody is not suitable for immunoprecipitation of native c-Myc protein. Flow cytometry: Intracellular or extracellular staining, depending on particular expression. Recommended dilution: 1-4 μg/ml.Western blotting: Recommended dilution: 0,5-2 μg/ml; positive control: c-Myc tagged protein.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 9E10
• Immunogen: Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
• Target: c-Myc tag
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

bHLH, MRTL, MYCC

Detection of transfected LST-1-c-Myc in HEK-293 cells (red) compared with nontransfected HEK-293 cells (black) using mouse monoclonal anti-c-Myc (9E10) purified, GAM-APC.

Quick Database Links

Gene Symbol

c-Myc tag

UniProt

P01106

NCBI Gene ID

4609