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Anti-c-Myc tag Purified
Catalog Number: orb43629
| Catalog Number | orb43629 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to c-Myc. |
| Clonality | Monoclonal |
| Clone Number | 9E10 |
| Tested applications | ELISA, FC, ICC, IP, WB |
| Reactivity | All, Human |
| Isotype | Mouse IgG1 |
| Immunogen | Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | c-Myc tag |
| Entrez | 4609 |
| UniProt ID | P01106 |
| RRID | AB_10990428 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-c-Myc antibody, anti-Cellular myelocytomatosi Read more... |
| Note | For research use only |
| Application notes | Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive tissue: perfused brain sections, liver, spleen. Immunoprecipitation: Recommended dilution: 1-5 μg/ml; this antibody is not suitable for immunoprecipitation of native c-Myc protein. Flow cytometry: Intracellular or extracellular staining, depending on particular expression. Recommended dilution: 1-4 μg/ml.Western blotting: Recommended dilution: 0,5-2 μg/ml; positive control: c-Myc tagged protein. |
| Expiration Date | 12 months from date of receipt. |
Detection of transfected LST-1-c-Myc in HEK-293 cells (red) compared with nontransfected HEK-293 cells (black) using mouse monoclonal anti-c-Myc (9E10) purified, GAM-APC.
