c-Myc tag Antibody (Biotin)

SKU: orb222741

Description

Mouse monoclonal antibody against c-Myc conjugated to Biotin

Images & Validation

• Tested Applications: ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB
• Reactivity: All, Human
• Application Notes:
  Flow cytometry: Intracellular or extracellular staining, depending on particular expression. Recommended dilution: 1-5 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 9E10
• Immunogen: Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
• Target: c-Myc tag
• Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin

Storage & Handling

• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

bHLH, MRTL, MYCC

Separation of cells stained using anti-c-Myc tag (9E10) Biotin antibody (concentration in sample 5 μg/ml, Streptavidin PE, red-filled) from cells unstained by primary antibody (Streptavidin PE, black-dashed) in flow cytometry analysis (surface staining) of LST-1-c-Myc transfected HEK-293 cells.

Flow cytometry intracellular staining pattern of LST-1-c-Myc transfected HEK-293 cells stained using anti-c-Myc tag (9E10) Biotin antibody (concentration in sample 5 μg/ml, Streptavidin PE).

Quick Database Links

Gene Symbol

c-Myc tag

UniProt

P01106

NCBI Gene ID

4609