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Catalog Number | orb345393 |
---|---|
Category | Antibodies |
Description | MYC Epitope Tag antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Isotype | IgG |
Immunogen | This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide. The sequence corresponds to amino acids 410-419 of human c-Myc. |
Concentration | 1.0 mg/ml |
Dilution range | ELISA: 1:135,000, IHC: User Optimized, WB: 1:500 - 1:5,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This affinity purified antibody is directed against human c-Myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
Conjugation | Unconjugated |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Rabbit anti-MYC Epitope Tag Antibody, Rabbit anti- Read more... |
Note | For research use only |
Application notes | Anti-Myc has utility to detect the fusion protein of the Myc epitope cloned along with the target gene. As such, anti-Myc/Myc can be used to identify fusion proteins containing the Myc epitope. The antibody recognizes the Myc tag fused either to the AMINO- or CARBOXY- termini of targeted proteins. This antibody was tested by ELISA and western blotting and was tested against both the immunizing peptide and Myc-tagged recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of recombinant protein present in ~35 ug of lysate using MYC Epitope Tag antibody
Western blot analysis of recombinant protein.
IF, IHC-Fr, IHC-P, IP, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, IP, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, IP, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, IP, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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