Beta-tubulin Antibody

• Catalog Number: orb393345
• Category: Antibodies
• Description: The Beta-tubulin Antibody is suitable for IHC, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence of human Beta-tubulin. The exact sequence is proprietary. Purification: Affinity chromatography
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Gallus, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Zebrafish
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 0.2% rAlbumin, 30% glycerol, and 0.01% sodium azide.
• Purification: Affinity chromatography
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Beta-tubulin. The exact sequence is proprietary.
• UniProt ID: Q9ERD7, Q4QRB4, Q13509
• Tested applications: IHC, IP, WB
• Dilution range: WB: 1:5000:10000, IHC-P: 1:100:200, IP: 1:100:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain;
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 246118, 10381, 22152

References

Poonam Dhiman 1, Rajneesh Kumar 1, Damanpreet Singh Neuronal nitric oxide synthase activation by tadalafil protects neurological impairments in a zebrafish larva model of hyperammonemia Life Sci ., (2024)

PubMed: 39710060

Western blot analysis of Beta-tubulin expression in Hela (A), mouse kidney (B), rat heart (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 52 kD)

Immunohistochemical analysis of Beta-tubulin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunoprecipitation of Beta-tubulin from 0.5 mg mouse brain whole cell extract lysate, using Anti-Beta-tubulin Antibody.