Beta-tubulin Antibody

SKU: orb393345

Description

The Beta-tubulin Antibody is suitable for IHC, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence of human Beta-tubulin. The exact sequence is proprietary. Purification: Affinity chromatography

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB: 1:5000:10000, IHC-P: 1:100:200, IP: 1:100:200
• Reactivity: Gallus, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Beta-tubulin. The exact sequence is proprietary.
• Purification: Affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 0.2% rAlbumin, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III

Western blot analysis of Beta-tubulin expression in Hela (A), mouse kidney (B), rat heart (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 52 kD)

Immunohistochemical analysis of Beta-tubulin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunoprecipitation of Beta-tubulin from 0.5 mg mouse brain whole cell extract lysate, using Anti-Beta-tubulin Antibody.

Quick Database Links

UniProt

Q4QRB4, Q9ERD7, Q13509

NCBI Gene ID

22152, 10381, 246118

References

Poonam Dhiman 1, Rajneesh Kumar 1, Damanpreet Singh Neuronal nitric oxide synthase activation by tadalafil protects neurological impairments in a zebrafish larva model of hyperammonemia Life Sci ., (2024)

PubMed: 39710060