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Anti-Beta-tubulin Antibody

Catalog Number: orb393042

DispatchUsually dispatched within 5-10 working days
$ 100.00
Catalog Numberorb393042
CategoryAntibodies
DescriptionRabbit polyclonal antibody to Tubulin beta 3.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityA. thaliana, Human, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Beta-tubulin. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500:1000, IHC-P: 1:100:200, IF/ICC: 1:100:500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetTUBB3
Entrez22152, 246118, 10381
UniProt IDQ4QRB4, Q9ERD7, Q13509
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti-beta 3 tubulin antibody, anti-beta-4 antibody
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-Beta-tubulin Antibody

Western blot analysis of Beta-tubulin expression in HEK293T (A), A375 (B), DLD (C), HepG2 (D), mouse brain (E), rat brain (F), rat testis (G) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 55 kD)

Anti-Beta-tubulin Antibody

Immunohistochemical analysis of Beta-tubulin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Beta-tubulin Antibody

Immunofluorescent analysis of Beta-tubulin staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    FC,  ICC,  IF,  IHC,  WB

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