Anti-BCKDHA Antibody

• Catalog Number: orb412883
• Category: Antibodies
• Description: Rabbit polyclonal antibody to BCKDHA
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human BCKDHA
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: BCKDHA
• Entrez: 25244, 593
• UniProt ID: P11960, P12694, P50136
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-2-oxoisovalerate dehydrogenase subunit alpha,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of BCKDHA expression in SGC7901 (A), mouse liver (B), mouse spleen (C), rat liver (D), rat spleen (E) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 50; 40 kD)

Immunofluorescent analysis of BCKDHA staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.