BCKDHA Antibody (C-term)

• Catalog Number: orb37075
• Category: Antibodies
• Description: Purified rabbit polyclonal antibody.
• Target: BCKDHA
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Immunogen: Synthetic Peptide
• UniProt ID: P12694
• MW: 50471
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50
• Antibody Type: Primary Antibody
• Clone Number: RB20924
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-2-oxoisovalerate dehydrogenase subunit alpha
• Research Area: Cell Biology
• Note: For research use only
• NCBI: NP_000700.1
• Expiration Date: 12 months from date of receipt.

Western blot analysis of BCKDHA Antibody (C-term) in Jurkat cell line lysates (35 ug/lane). BCKDHA (arrow) was detected using the purified Pab.

BCKDHA Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with BCKDHA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.