Anti-ATG4D Antibody

• Catalog Number: orb515194
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ATG4D
• Target: ATG4D
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATG4D. The exact sequence is proprietary.
• UniProt ID: Q86TL0, Q8BGV9
• Tested applications: IH, IP, WB
• Dilution range: WB: 1:500-1000, IHC-P: 1:50-100, IP: 1:10-100
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: APG4D; AUTL4; Cysteine protease ATG4D; AUT-like 4
• Note: For research use only
• Entrez: 235040, 84971

Western blot analysis of ATG4D expression in Hela (A), A549 (B), PC12 (C), CT26 (D) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 52 kD)

Immunohistochemical analysis of ATG4D staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.