ATG4D Antibody (Center)

• Catalog Number: orb1933458
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This ATG4D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from the Central region of human ATG4D.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q86TL0
• MW: 52922 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Antibody Type: Primary Antibody
• Clone Number: RB7565
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cysteine protease ATG4D, 3422-, AUT-like 4 cystein
• Note: For research use only
• NCBI: NP_116274.3, NP_001268433.1

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-APG4D Pab in mouse kidney tissue lysate. APG4D (arrow) was detected using the purified Pab.

APG4D Antibody (L235) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the APG4D antibody detected the APG4D protein (arrow).

Western blot analysis of anti-hAPG4D-L235 Pab in 293 cell line lysates transiently transfected with the APG4D gene (2 ug/lane). hAPG4D-L235 (arrow) was detected using the purified Pab.