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ATG4D Antibody (Center)
Description
Images & Validation
−| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000, IHC-P - 1:100-500 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Porcine |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 52922 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−ATG4D Antibody [orb515194]
IHC, IP, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μl, 30 μl, 100 μl, 200 μl

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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-APG4D Pab in mouse kidney tissue lysate. APG4D (arrow) was detected using the purified Pab.

APG4D Antibody (L235) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the APG4D antibody detected the APG4D protein (arrow).

Western blot analysis of anti-hAPG4D-L235 Pab in 293 cell line lysates transiently transfected with the APG4D gene (2 ug/lane). hAPG4D-L235 (arrow) was detected using the purified Pab.
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Protocol Information
ATG4D Antibody (Center) (orb1933458)
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