ATG4D Antibody (N-term)

• Catalog Number: orb29416
• Category: Antibodies
• Description: Purified rabbit polyclonal antibody.
• Target: ATG4D
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Immunogen: Synthetic Peptide
• UniProt ID: Q86TL0
• MW: 52922
• Tested applications: IF, IHC-P, WB
• Dilution range: IF/ICC: 1:200, WB: 1:1000, IHC-P: 1:50-100
• Antibody Type: Primary Antibody
• Clone Number: RB14788
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-ATG4D, Cysteine protease ATG4D, APG4D antibod
• Research Area: Cancer Biology, Cardiovascular Research, Cell Biol
• Note: For research use only
• NCBI: NP_001268433.1, NP_116274.3

ATG4D Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ATG4D Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ATG4D Antibody (N-term) western blot analysis in mouse liver tissue lysates (35 ug/lane). This demonstrates the ATG4D antibody detected the ATG4D protein (arrow).

Fluorescent image of U251 cells stained with ATG4D (N-term) Antibody. U251 cells were treated with Chloroquine (50 μM, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ATG4D (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). ATG4D (N-term) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.